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H (1963) Chromatographlc determination of amino acids by the use of automatic recording equrpment. , and Stem W H (1958) Chromatography of ammo acids on sulfonated polystyrene resms Anal Chem. 30, 1185-1190. M. E , and Bachmann C (1972) Nyhan W. , Hornbeck Amplcrllm and ammo acrd chromatography. 1. Pedzat 81, 1215-1216. Partridge S M (1949a) Drsplacement chromatography on synthetic ronexchange resms 3 Fractionatron of a protein hydrolyzate Blochem J 44, 521-527 Automated Amino Acid Analysis 25 Partrrdge S M.

The technique of partial thawing to a waxy consistency for drssectron should be strictly followed. GC of Amino Acids 33 However, even rf the specimen is instantly frozen when removed, biochemrcal changes may have already occurred during surgery and brain death may introduce artifacts. To mmimrze these problems, in VIVO sampling techniques that permit local brain perfusions have been developed. , 1984). To date, these techniques have been applied only to laboratory animals. 5. Sample Selection and Preparation Amino acid contents have been studied in a variety of brologrcal samples.

Lnd Eng Chem 36, 604609 Felix A M. and Terkelsen G. (1973) Total fluorometric ammo acid analysis using fluorescamme Arch Bzochem Blophys 157, 177-182, Fmkelstem J D , Kyle W E , and Martm J J (1975) Abnormal methiorune adenosyltransferase in hypermethionmemia Bzochem Bzophys Res Commun 66, 1491-1497 Gaul1 G E, and Tallan H H. (1974) Methionme adenosyltransferase deficrency. enzymatic defect New associated with hypermethionmemra Sczence 186, 59-60 Automated Amino Acid Analysis 23 Gaul1 G E , Bender A N Vulov~c D , Tallan H.

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