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Instruments that use filters are called fluorometers. Those which use monochromators are spectrofluorometers; these allow scanning of the wavelengths and hence determination of the optimal wavelengths of both excitation and emission. Lasers generally provide monochromatic light, so an excitation wavelength selector (discriminator 1) is not needed in this case. Sample Cuvettes are normally 1 cm in path length and made of quartz (UV region) or glass (Vis region) for laboratory instruments. At least two adjacent faces will be transparent.

E. thermocouples, thermistors and bolometers. A thermocouple is based on the use of two different conductors connected by a junction. When a temperature difference is experienced at the junction, a potential difference can be measured. A series of thermocouples together is called a thermopile. Thermistors and bolometers are based on a change in resistance with temperature. They have a faster response time than thermocouples. With a Fourier Transform IR (FTIR), where rapid response and improved sensitivity is key, lead sulfide and InGaAs detectors are used as for NIR.

The UV absorption spectrum and fluorescence excitation spectrum of a molecule often occur at similar wavelengths. 22 Some examples of compounds that readily fluoresce due to their rigid, planar structures. 23 Excitation (1) and emission (2) spectra of aspirin in the solid phase (Reprinted from Analytica Chimica Acta. B. , ‘Solid-phase fluorescence spectroscopy for the determination of acetylsalicylic acid in powdered pharmaceutical samples’, 523(1), 49–5221. Copyright 2004, with permission from Elsevier).

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