By Csaba Horvath, John G. Nikelly
content material: High-resolution nanotechnique for separation, characterization, and quantitation of micro- and macromolecules : capillary electrophoresis / Noberto A. Guzman, Luis Hernandez, and Shigeru Terabe --
purposes of capillary quarter electrophoresis to quality controls / R.G. Nielsen and E.C. Rickard --
research of cyclic nucleotides through capillary electrophoresis utilizing ultraviolet detection / Luis Hernandez, Bartley G. Hoebel, and Noberto A. Guzman --
On-column radioisotope detection for capillary electrophoresis / Stephen L. Pentoney, Jr., Richard N. Zare, and Jeff F. Quint --
innovations for an analytical exam of organic prescribed drugs / Eleanor Canova-Davis, Glen M. Teshima, T. Jeremey Kessler, Paul-Jane Lee, Andrew W. Guzzetta, and William S. Hancock --
Analytical chemistry of healing proteins / R.M. Riggin and N.A. Farid --
development and experimental validation of protein impurity immunoassays for recombinant DNA items / Vincent Anicetti --
minimal variance purity keep an eye on of preparative chromatography with simultaneous optimization of yield : an online species-specific detector / Douglas D. Frey --
Amino acid sequence-mass spectrometric analyses of mating pheromones of the ciliate Euplotes raikovi / R.A. Bradshaw, S. Raffioni, P. Luporini, B. Chait, T. Lee, and J. Shively --
Micropellicular sorbents for speedy reversed-phase chromatography of proteins and peptides / Krishna Kalghatgi and Csaba Horváth --
Displacement chromatographic separations on [beta]-cyclodextrin-silica columns / Gyula Vigh, Gilberto Quintero, and Gyula Farkas.
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Additional info for Analytical Biotechnology. Capillary Electrophoresis and Chromatography
Although the capillary column seems to be very durable (because of the externally coated polyimide polymer), a region of the column (approximately 1- to 2-cm) is burned-off allowing it to become transparent. This transparent region is centered and aligned to allow the passage of a light beam for detection and quantitation of the analytes under study. Careful handling conditions of the capillary is recommended at this stage (since the new uncoated region become very fragile). The accomodation of various sizes and chemistries of the capillaries makes the system very convenient for the preparation of different capillary column i advance well makin th system very simple (fo channels, allowing the passage of a thermoregulated fluid.
Chromatographia 1987, 24, 527. 83. P. The 41st Pittsburgh Conference & Exposition on Analytical Chemistry and Applied Spectroscopy. New York, New York, 5-8 March 1990. Abstract 706. 84. W. J. Chromatogr. 1988, 438, 23. 85. E. First Symposium of The Protein Society. San Diego, California, 9-13 August 1987. Abstract 823. ; ACS Symposium Series; American Chemical Society: Washington, DC, 1990. 35 Chapter 2 Applications of Capillary Zone Electrophoresis to Quality Control R. G. Nielsen and E. C. Rickard Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285 Performance parameters were established for the use of capillary zone electrophoresis (CZE) in the characterization of biosyntheti huma growth hormon (hGH) CZE distinguished betwee of hGH and corresponding fragments produced from most of its chemically similar impurities and degradation products.
Biochem. 1989 177 20-26 16. Grossman, P. ; Colburn Riggin, R. ; Sittampalam 1989, 61, 1186-1194. 17. Nielsen, R. ; Riggin, R. ; Rickard, E. C. J. , 1989, 480, 393-401. 18. Nielsen, R. ; Rickard, E. C. J. , submitted. 19. Moring, S. ; Colburn, J. ; Grossman, P. ; Lauer, Η. H. LC-GC 1990, 8, 34-46. 20. Grossman, P. ; Wilson, K. ; Lauer, Η. H. Anal. Biochem. 1988, 173, 265-270. 21. Rose, D. ; Jorgenson, J. W. Anal. Chem. 1988, 60, 642-648. 22. Rose, D. ; Jorgenson, J. W. J. Chromatogr. 1988, 447, 117-131.