By Wai Tak Law, Naim Akmal, Arthur Usmani

Containing smooth options within the layout and alertness of sensing units, Biomedical Diagnostic technology and know-how is a state of the art resource for analytical, natural, medicinal, actual, floor, and colloid chemists and biochemists; molecular and phone biologists; geneticists; utilized and business microbiologists; virologists; endocrinologists; digital, fabrics, chemical, and mechanical engineers and bioengineers; pharmacists; and upper-level undergraduate and graduate scholars in those disciplines.

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J. Cunningham. Introduction to Bioanalytical Sensors. Wiley, New York, 1998. 6. B. Eggins. Biosensors: An Introduction. Wiley-Teubner, New York, 1996. 7. F. W. Scheller, F. Schubert, J. Fedrowitz , eds. Frontiers in Biosensorics 1 & 11. Birkha¨user Verlag, Basel, 1997. 8. J. S. Sidwell, G. A. Rechnitz. Biotechnol. Lett. 7:419, 1985. 9. C. P. Price, D. J. Newman, eds. Principles and Practices of Immunoassay. 2nd ed. Macmillan Reference, London, 1997. 10. G. Kohler, C. Milstein. Nature 256:495, 1975.

Although the sensor surface can in principle be renewed by cleaving the antibody–antigen bonds in acidic media, it is tedious to do and often results in the loss of antibody activity. A number of immunsensor designs have been used to overcome these challenges. One of these has been to bind the antibodies to a membrane, which is then placed over the electrode [43]. Detection in these immunosensors is based on a competitive assay format, as illustrated in Figure 8. Here, an aliquot of enzyme-labeled analyte (Ag*) of known concentration is added to the sample for competitive binding to the antibody.

Lemmonier, J. C. Klein, Y. Hu, G. S. Wilson. Biosens. Bioelectron. 12:1061, 1997. 30. V. Thome´-Duret, G. Reach, M. N. Gangnerau, F. Lemonnier, J. C. Klein, Y. Zhang, Y. Hu, G. S. Wilson. Anal. Chem. 68:3822, 1996. 31. E. Csoregi, D. W. Schmidtke, A. Heller. Anal. Chem. 67:1240, 1995. 32. J. G. Wagner, D. W. Schmidtke, C. P. Quinn, T. F. Fleming, B. Bernacky, A. Heller. Proc. Natl. Acad. Sci. USA 95:6379, 1998. 33. J. C. Armour, J. Y. Lucisano, B. D. McKean, D. A. Gough. Diabetes 39:1519, 1990.

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