By Anthony P. Hollander, Paul V. Hatton
Professional laboratory researchers describe in a typical layout the entire varied laboratory equipment had to practice state of the art tissue engineering. subject matters diversity from the synthesis, processing, and characterization of particular biomaterials, in the course of the winning use of scaffolds within the engineering of tissues, to options helpful in comparing the organic caliber of scaffold-engineered tissues. issues of distinct curiosity comprise the incorporation of organic molecules into scaffold biomaterials and using quite a lot of equipment and strategies to generate a finished description of cell-polymer build caliber.
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Additional resources for Biopolymer Methods in Tissue Engineering
2. 28 g of sodium bicarbonate, and 1 g of citric acid (see Note 7). 3. Homogenize the entire mixture in a mixer 4. Stratify the paste using a mangle-device consisting of two rollers that turn in opposite directions with an adjustable distance between the two. 5 cm. 3 cm), the paste is forced between the rollers together with a strip of silicone sheet which acts as a support to the layer of paste just formed. 5. , 1 cm in diameter). 6. Remove from the silicone. 03/Soranzo/25-40 34 09/25/2003, 11:19 AM Hyaluronan-Based Polymers 35 7.
12. 50-mL screw-capped centrifuge tubes. 13. ). 14. Lyophilization chamber (to accommodate 50-mL centrifuge tube). 15. Sharp, straight-edged blades such as disposable microtome blades. 16. Circular punch with 5–10-mm diameter. 17. Dessicator. 18. Ethanol, 100% and 50, 60, 70, and 95% in distilled water. 19. Antibiotic-antimycotic solution in phosphate-buffered saline (PBS): 20,000 U/mL penicillin, 20 mg/mL streptomycin, and 50 µg/mL amphotericin B (Sigma A9909 antibiotic-antimycotic solution, 2×).
And Smidsrod, O. (1997) In vitro degradation rates of partially N-acetylated chitosans in human serum. Carbohydr. Res. 299(1–2), 99–101. 04/Elder/41-48 47 09/25/2003, 11:21 AM 48 Elder, Nettles, and Bumgardner 12. Klokkevold, P. , Sung, E. , and Bertolami, C. N. (1999). The effect of chitosan (poly-N-acetyl glucosamine) on lingual hemostasis in heparinized rabbits. J. Oral Maxillofac. Surg. 57(1), 49–52. 13. Muzzarelli, R. A. (1999) Clinical and biochemical evaluation of chitosan for hypercholesterolemia and overweight control EXS 87, 293–304.