By A. Fiechter (auth.)

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4. Growth factors. These are mostly vitamins =- organic compounds that are supplied in low quantities but cannot be synthesized by the cell; in other cases, growth factors may limit growth kinetics. In medium preparation, the problem with organic growth factors is their susceptibility to degradation during heating. Physical and Chemical Parameters of Microbial Growth 43 Consequently, such growth factors must be dissolved and (filter-)sterilized separately and then added only after the bulk of the media has been prepared.

Very often, defined media for yeast studies are actually controlled by fin unknown limitation of trace elements such as iron, magnesium or manganese that override the effects of carbon limitation. The results of this inconsistency can be rather drastic, as demonstrated by Rieger 53~. When an improved medium with perfect C-limitation was used in regulatory studies of Saccharomyces cerevisiae, differences between earlier experiments became obvious. The classical theory of control of respiration by glucose turned out to be erroneous.

15a. Performance of TORUS 100 1in biotesting with non-Newtonian fluids. 3 The limitations in mass transfer by low power input and increased viscosity (G, J) of the fluid are well demonstrated by these experiments; b. Biotesting of FBT 5 I (Gschwend 20)). 5 9/~. Plot rpm P/W Wl-Z D F i000 1000 5 5 3 stirrers 1 stirrer (upper 2 removed) The drastic reduction of biomass formation in presence of xanthan (o-o-) is a characteristic of the FBT-system due to the sharp reduction of turbulence in the bulk of the fluid is not used, operation and maintenance, including sterility control, appear to be simple and inexpensive.

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