By V. G. Berezkin

During this two-part monograph, the writer describes sleek equipment for the swift column liquid chromatography of excessive- and medium-molecular-weight compounds of organic foundation, i.e. proteins, peptides, enzymes, nucleic acids, poly- and oligonucleotides, poly- and oligosaccharides, complicated biopolymers and biooligomers similar to viruses, bacteriophages, ribosomes and glycoconjugates, in addition to another compounds similar to immunomodulators. the fabric is contained in components: half A facing common chromatographic idea, ideas, fabrics and strategies; and half B facing the separation of person compound periods and containing a sign in of chromatographed components and a full-title bibliography. not just is that this a really expert, certain treatise on chromatographic recommendations, it additionally offers a huge, balanced assessment of speedy separation of all recognized vital biopolymers and biooligomers, either uncomplicated and complicated, and in addition of a few synthetically ready and pharmaceutically very important biooligomers. moreover, it offers an creation to the applying of HPLC to the examine of the constitution of those elements.

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This method and device also make it possible to obtain a solution of diazoethane in diethyl ether when N-ethyl-N-nitroso-N-nitroguanidine is used. Ethers of acids can also be formed by reaction with diazomethane in a chromatograph in a special quartz reactor filled with quartz-wool and placed before the chromatographic column [ 1011 . The sample and the diazomethane solution are placed in the reactor. Esterification is completed in 18 sec. Diazomethane is also used for methylenation under the conditions of diazomethane photolysis.

The stability of the compounds obtained must be sufficiently high. , TMS) are sensitive to moisture. A useful method of increasing substantially the stability of derivatives during chromatographic separation that has been applied in the analysis of volatile chelates of a number of elements can be mentioned. S 32 0 I 10 I 20 I 30 I 40 Tlme (min) Fig. 9. Chromatogram of the separation of tritluoroacetylacetonates of rare earth elements in a flow of carrier gas with trifluoroacetylacetonate. From ref.

From ref. 53. Hence the application of the CFD method makes it possible in a number of instances to improve separations as a result of the formation of derivatives with greater differences in chromatographic properties. Unfortunately, this area of application of the CFD method has not yet been sufficiently developed. There is no doubt that its further development will help in the solution of many practical problems. The third positive feature of CFD methods is a substantial improvement in the quantitative characteristics of analytical determinations.

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