By M. A. Hayat

Correlative Microscopy In Biology

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17. C o m b i n e d L M / S E M m i c r o g r a p h s of a n o r m a l i n t e r m e d i a t e cervical cell p r e p a r e d as in Fig. 15, s h o w i n g the basal side, a, L M a b s o r p t i o n image, b , L M fluorescence image, c, S E M i m a g e . F u r r o w s c a n be seen as g r o o v e s and microvilli are p r e s e n t , d, Detail of the surface s t r u c t u r e . 45 2. L M / S E M i n a C o m b i n e d I n s t r u m e n t 10- ΓτίΤΤ#Ττ- π ,m , π ρ Fig. 18. D N A m e a s u r e m e n t s of cervical epithelial cells from c a r c i n o m a lesion after the p r e p a r a t i o n m e t h o d d e s c r i b e d in Fig.

1983). S c a n n i n g e l e c t r o n m i c r o s c o p y in c y t o d i a g n o s i s . Scanning Electron Microsc. 3 , 1307. Geissinger, H . D . (1971). C o r r e l a t e d light optical and scanning e l e c t r o n m i c r o s c o p y of G r a m s m e a r s of b a c t e r i a and paraffin sections of c a r d i a c m u s c l e . J. Microsc. {Oxford) 9 3 , 109. Geissinger, H . D . (1974). A p r e c i s e stage a r r a n g e m e n t for correlative m i c r o s c o p y for specim e n s m o u n t e d on glass slides, stubs or E M grids.

3-D I m a g i n g T e c h n i q u e s with C S L M Visualization of M i t o c h o n d r i a Visualization of C h l o r o p l a s t s Conclusion and Outlook References INTRODUCTION Confocal scanning laser microscopy (CSLM), as recently come into being, possesses a number of specific advantages in comparison with conventional microscopy. The most important of these are an extended dynamic range and a basically improved resolving power, both transverse as well as along the optical axis. The manner in which data are collected in this instrument is very suitable for storage, display, and subsequent data processing by digital computer.

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