By Paul D. van Helden (auth.), Prof. Dr. Jörg T. Epplen, Dr. Thomas Lubjuhn (eds.)

This booklet displays functional ways to answering quite a few organic and scientific questions utilizing DNA fingerprinting and genetic profiling in a wide experience that still comprises statistical overview of the knowledge. it's been written for biology, biochemistry and clinical scholars, graduates, postdoctoral fellows, educational lecturers and for all researchers within the theoretical and technologies at any place genetic id and dating research are required.

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Theor Appl Genet 86: 449-457 37 27 Weising K, Ramser D, Kaemmer D, Kahl G (1994) Multilocus DNA fingerprinting and genetic relatedness in plants: a case study with banana and tomato. In: B Schierwater, B Streit, GP Wagner, R DeSalle reds): Molecular ecology and evolution: approaches and applications. Birkhiiuser Verlag, Basel, 45-59 28 Van Heusden AW, Van de Voort JR, Bachmann K (1991) Oligo-(GATA) fingerprints identify clones in asexual dandelion Taraxacum (Asteraceae). Fingerprint News 3: 13-15 29 Piquot Y, Saumitou-Laprade P, Petit D, Vernet P, Epplen JT (1996) Genotypic diversity revealed by allozymes and oligonucleotide DNA fingerprinting in French populations of the macrophyte Sparganium erectum.

Mol Ecol7: 465-474 Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Homes M, Frijters A, Pot J, Peleman J, Kuiper M et al (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23: 4407-4414 Taberlet P, Gielly L, Pautou G, Bouvet J (1991) Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Bioi 17: 11051109 Demesure B, Sodzi N, Petit RJ (1995) A set of universal primers for amplification of polymorphic non-coding regions ofmitochondrial and chloroplast DNA in plants.

2 mM dNTPs, 38 ng of Eco + XI X2 X3 5' digoxigenine-Iabelled primer (dig 5'GACTGCGTACCAATTC XI X2 X3 ), 30 ng of Mse + Y1 Y2 Y3 primer (5'-GATGAGTCCTGAGTAA Y1 Y2 Y3 ), ultrapure water. Xz X3 and YZY3 are arbitrary chosen base pairs, Xl and Y1 are the bases as in the preamplification]. 4. 7 °C at each cycle and 23 cycles at 94 0c/ 30 sec, 56 °C/30 sec, 72 °C/60 sec. Protocol 5d Electrophoresis 1. 0),10 mg of bromophenol blue, 10 mg of xylene cyanol] to the reaction. 2. 4-mm spacers and shark tooth combs.

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