By Michael P. Wilson, Kama Z. Guluma, Stephen R. Hayden
A realistic advisor to figuring out and navigating the original demanding situations confronted by means of physicians and different execs who desire to adopt examine within the ED or different acute care setting.
targeting the hyper-acute and acute care atmosphere and gratifying closely-related needs:
1) the necessity for even pro researchers to appreciate the explicit logistics and problems with doing examine within the ED; and a couple of) the necessity to teach clinically lively physicians in examine methodology.
This new textual content isn't designed to be a fancy, encyclopedic source, yet in its place a concise, easy-to-read source designed to show key “need-to-know” details inside of a finished framework. geared toward the busy mind, both as a sit-down learn or as a selectively-read reference advisor to fill in wisdom gaps, chapters are brief, compartmentalized, and are used strategically in the course of the textual content with a view to introduce and body recommendations. This structure makes it effortless - or even exciting - for the study beginner to combine and take up thoroughly new (and in general dry) fabric.
The textbook addresses elements of feasibility, potency, ethics, records, defense, logistics, and collaboration in acute study. total, it delivers entry for the professional researcher trying to find out about acute examine to emphatically combine studying issues into his or her wisdom base.
As the ED is the first atmosphere for hyper-acute and acute care, and hence a first-rate web site for comparable scientific trial recruitment and interventions, the publication provides particular logistical study demanding situations that researchers from any self-discipline, together with physicians, examine nurse coordinators, research displays, or companions, have to comprehend in an effort to be successful.
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Additional info for Doing Research in Emergency and Acute Care : Making Order Out of Chaos
Peptides, proteins, oligonucleotides, and even nanoparticles have been transduced efficiently into Julie Audet, William L. ), Stem Cells in Regenerative Medicine, Methods and Protocols, vol. com 43 44 Manceur and Audet different cell types (3–5). From past studies, it is now clear that the uptake depends on the CPP and the cell type used (6). Other important factors include the concentration of the CPP and the incubation time. Since there are many parameters to optimize, to maximize the cellular uptake, flow cytometry with fluorescentlylabeled constructs is an attractive approach because of its high-throughput and multiplex capabilities.
Those include the type of cells targeted, the type of CPP used, and the properties of the cargo. For this reason, it is important to have a means to quantitatively assess the transduction efficiency of specific constructs in the cell type of interest in order to select the best vector for a specific application. In this chapter, we describe a method to measure the uptake of HIV transactivator of transcription (TAT) and the homeobox protein Antennapedia (Antp) constructs in primary hematopoietic progenitor cells and hematopoietic cell lines.
Can be used. 5. All bake times (pre-, soft-, post-, and hard-bake) are dependent on the thickness of the resist layer and need to be empirically optimized for a given thickness. 6. Strength of the resist layer and adhesion to the Si wafer is dependent on bake and exposure times. Allowing the wafers to cool slowly will prevent cracking and strengthen the resist. Increasing post- and hard-baking times after developing will also improve adhesion and strength. 7. After drying the stamp, it is recommended to print onto the substrate as soon as possible.