By Nagy A. Habib
Nagy Habib and a group of scientific laboratorians and physicians describe the big variety of strong new laboratory equipment at present getting used for investigating and treating hepatocellular carcinoma. The booklet makes a speciality of gene remedy ways, together with using such vectors as lipids, such viruses as adenovirus and baculovirus, and virus detection review utilizing electron microscopy. It offers preclinical and scientific information at the killing of melanoma cells utilizing tumor-suppressor genes, antisense compounds to progress components, immunotherapy (remove gene), or virus-directed enzyme prodrug treatment. Hepatocellular Carcinoma: tools and Protocols bargains experimental and medical researchers a wealthy resource of either simple technology and medical info on modern optimum use of gene remedy to regard and deal with sufferers being affected by hepatocellular carcinoma.
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Extra info for Hepatocellular Carcinoma. Methods and Protocols
Inactivation of the p53 function through mutation or interaction with viral oncoproteins, therefore, is a common event during human carcinogenesis, which may provide a selective advantage for clonal expansion of preneoplastic and neoplastic cells. Given the nature of p53 as a common target for various types of oncoviral proteins-mediated neoplastic transformation, including SV40 T antigen, E1A and E1B of adenovirus, and E6 and E7 of oncogenic HPV (68), it is reasonable to speculate that p53 may also be the target for HBx.
3% skim milk in PBS. 5% Tween-20 in PBS. Anti-NS3 monoclonal antibody (4A-3). Anti-p53 monoclonal antibody (Ab-1 [clone 421]). Peroxidase-labeled goat anti-mouse IgG (MBL). Enhanced chemiluminescence (ECL) (Amersham Pharmacia Biotech, Buckinghamshire, UK). 8. Immunoprecipitation Analysis 1. 2. 3. 4. 5. 6. 7. 25 µCi of 35S-translabel (Amersham). 5). Anti-NS3 monoclonal antibody (4A-3). Anti-p53 monoclonal antibody (Ab-1 [clone 421]). Anti-FLAG monoclonal antibody (M2, Kodak). Protein G/Protein A–sepharose (Calbiochem).
Hepatology. 10, 346–348. 56. , Kok, T. , et al. (1993) Double blind clinical trial of antiandrogen therapy versus placebo in unresectable hepatocellular carcinoma (abstract). Am. Soc. Clin. Oncol. 12, 193. 57. , and Manousos, O. N. (1998) Treatment of hepatocellular carcinoma with octreotide: a randomised controlled study. Gut. 42, 442–447. 58. Muto, Y. , Takasaki, K. , et al. (1996) Prevention of second primray tumors by an acyclic retinoid, polyprenoic acid, in patients with hepatocellular carcinoma.