By Andre P. De Leenheer, Willy Lambert

3rd version collects and examines the large proliferation of data on chromatographic research of fats and water soluble supplementations over the past decade. largely describes pattern guidance and ultimate size.

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Download PDF by Andre P. De Leenheer, Willy Lambert: Modern chromatographic analysis of vitamins

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Cis-trans conversion of β-carotene in FU-5 rat hepatoma cells was studied by HPLC analysis of cell extracts using a 5-µm Suplex PKB 100 column and a mobile phase of methanol/dichloromethane (97: 3) (230). Astaxanthin in Marine Organisms. The resolution of all-trans astaxanthin on a Pirkle covalent D-phenylglycine type column made in Japan has been described (231). A Pirkle covalent L-leucine column (5 µm) was used with a solvent mixture of hexane/THF to resolve the stereoisomers of all-trans astaxanthin (232).

IV. THIN-LAYER CHROMATOGRAPHY OF RETINOIDS AND CAROTENOIDS Although thin-layer chromatography (TLC) and open-column chromatography have played important roles historically in analysis of retinoids and carotenoids, both methods have been almost completely supplanted by HPLC. Paper chromatography has never been employed extensively for retinoid or carotenoid analysis. TLC is still useful for rapid assessment of HPLC methods, because it provides rapid analyses with different mobile phases in much less time than required for reequilibration of HPLC columns.

In a study by Hashimoto et al. (289), GC-MS was used to elucidate structures of rat metabolites of a synthetic retinoid, 4-[(5,6,7,8-tetrahydro-5,5,8,8tetramethyl-2-naphthyl)carbamoyl]benzoic acid. A GC-MS method using positive ion EI for quantitative analysis of the methyl ester of retinoic acid was reported by De Leenheer and Lambert (89) using deuterated retinoic acid as an internal standard. The sensitivity of GC-MS for retinoid analysis may be enhanced by using negative ion chemical ionization (NCI).

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